Part:BBa_K1830008:Experience

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BBa_K1830008 iGEM Concordia 2015

This part is a necessary requirement for transformation, conservation and replication of a plasmid transformed into L. lactis. We therefore tested by incorporating this part into our main construct. It was then electroporated into L. lactis and selected on chloramphenicol supplemented GM17 media. This test was repeated two times, one with a pSB1C3 plasmid containing only this part, and a second time with a pSB1C3 backbone containing the shuttle vector fused to our main construct. The two transformations were succesfull when plated on selective media. We then decided to check the transformation by extracting our plasmids out of L. lactis. The product was then electrophoresed as well as digested and ran on the same gel, and the functionality of the part was confirmed by the size of the insert and of the entire plasmid. No contamination was detected in the colonies we picked for analysis.

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